Download e-book for kindle: A Guide to Forensic DNA Profiling by Scott Bader, Allan Jamieson

By Scott Bader, Allan Jamieson

ISBN-10: 1118751523

ISBN-13: 9781118751527

The more and more arcane international of DNA profiling calls for that these desiring to appreciate not less than a few of it needs to discover a resource of trustworthy and comprehensible information.  Combining fabric from the winning Wiley Encyclopedia of Forensic Science with newly commissioned and up to date fabric, the Editors have used their very own wide event in felony casework the world over to bring together an informative consultant that might offer wisdom and thought-provoking articles of curiosity to somebody concerned or drawn to using DNA within the forensic context.

Following broad introductory chapters protecting forensic DNA profiling and forensic genetics, this accomplished quantity offers a considerable breadth of fabric covering:

  • Fundamental fabric  – together with assets of DNA, validation, and accreditation
  • Analysis and interpretation – together with, extraction, quantification, amplification and  interpretation of electropherograms (epgs)
  • Evaluation – together with combinations, low template, and transfer
  • Applications – databases, paternity and kinship, mitochondrial-DNA, natural world DNA, single-nucleotide polymorphism, phenotyping and familial searching
  • Court  - file writing, discovery, pass exam, and present controversies

With contributions from top specialists around the entire gamut of forensic technological know-how, this quantity is meant to be authoritative yet no longer authoritarian, informative yet understandable, and accomplished yet concise.  it is going to turn out to be a useful addition, and resource, for  scientists, lawyers,  academics, criminologists, and judges.

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Extra info for A Guide to Forensic DNA Profiling

Example text

STR Analysis STR markers are similar to the VNTR markers that were originally identifie and utilized in DNA fingerprinting and SLP profiling The difference between the classes of DNA marker lies in the length of the tandemly repeated DNA sequence. VNTRs contain 10–33-bp hypervariable repeat motifs [4] that must be observed over a size range of 4–20 kb [9] and SLP markers that are observed over a size range of 1–14 kb [10]. An STR marker repeat is composed of 1–6-bp repeat motifs [18], making the region of DNA that must be scrutinized very short (<1 kb).

Alternative DNA Markers Autosomal STR markers have become the most utilized ones in both forensic and paternity DNA profiling However, there are numerous alternative markers that can be interrogated when required. Another class of autosomal marker, the single nucleotide polymorphism (SNP), has been investigated for application to forensic casework and identificatio projects. SNPs, as the name suggests, are alterations of a single base pair and occur on average every few hundred base pairs throughout the human genome [50, 51].

PCR was firs applied to forensic DNA profilin for the investigation of the HLA-DQ-????1 gene, a polymorphic gene that encodes a human leukocyte antigen cell surface protein located in the major histocompatibility complex (MHC) class II region on chromosome 6 [14]. Two big breakthroughs occurred between the late 1980s and early 1990s that would form the basis of DNA profilin techniques that are recognized today. An alternative class of DNA marker, the microsatellite or short tandem repeat (STR) marker, was described by Weber et al.

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A Guide to Forensic DNA Profiling by Scott Bader, Allan Jamieson

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