By Prof. Dr. Michael L. G. Gardner, Prof. Dr. Klaus-Jürgen Steffens (auth.)
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Additional resources for Absorption of Orally Administered Enzymes
Hepatic Extraction and Biliary Excretion of Peptide and Protein Drugs As yet it is not known how well the Pz-peptide mentioned above will be absorbed when given orally. Two possible obstacles to its achieving significant oral bioavailability are hepatic extraction and biliary excretion. The role of hepatic extraction in the clearance of orally administered nonpeptide drugs is well known. But its potential role in the clearance of orally. administered peptide and protein drugs is not well appreciated.
8. By combining the antibody-mediated capture of bromelain and the use of a fluorogenic substrate, a practical limit of detection of 1 ng bromelain/rnl plasma could be achieved. When this approach was used for investigating the presence of bromelain in plasma samples, it turned out to be somewhat less sensitive than the ELISA. Clinical Study A randomized, controlled, double-blind study was performed with 19 healthy caucasian men, aged 18 to 45 years.
Concerning specificity and sensitivity, immunological methods have several advantages for the quantification of a protein. However, it cannot be ignored that an immune method quantifies the so-called immunereactive protein, that is to say, both the full-size antigen as well as any fragment able to be recognized by the specific antibody. A good antibody (high affinity and specificity) is the key to any sensitive immunological method. To meet this requirement, antibodies were raised in rabbits using purified bromelain as antigen.
Absorption of Orally Administered Enzymes by Prof. Dr. Michael L. G. Gardner, Prof. Dr. Klaus-Jürgen Steffens (auth.)