Advanced Techniques in Biological Electron Microscopy II: by G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.) PDF

By G. L. Nicolson (auth.), James K. Koehler Ph. D. (eds.)

ISBN-10: 3642668097

ISBN-13: 9783642668098

ISBN-10: 3642668119

ISBN-13: 9783642668111

The use of the time period "advanced" within the name of this booklet is a little bit ar­ bitrary and extremely a lot relative with recognize to time. Many innovations which have been thought of on the "cutting part" of ultrastructural method quite a few years in the past at the moment are rou tin ely utilized in a number of laboratories. you will cite freeze-fracture, cryothin sectioning, or certainly lots of the box of test­ ning electron microscopy as concrete examples. therefore using the time period "ad­ vanced ideas" has to be interpreted with reference to the current state-of-the-art, and turns out to be useful simply in informing the capability reader that this quantity isn't really a primer for use as an preliminary creation into simple organic elec­ tron microscopy. many fantastic volumes have stuffed that area of interest some time past few years, and it isn't meant that this modest ebook be a whole com­ pendium of the sphere. moreover, any restricted choice of papers on advanc­ ed strategies unavoidably displays the personal tastes and arbitrary whims of the editor, thereby except for many both very important systems which the an expert reader will with ease determine. the 1st quantity of this sequence seemed nearly 5 years in the past and illustrated thoughts which have been proposal to symbolize complex and but ba­ sically morphological equipment for gaining elevated ultrastructural informa­ tion from organic specimens. the current quantity, however, stresses thoughts which offer particular physicochemical facts at the speci­ mens as well as the structural information.

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Extra resources for Advanced Techniques in Biological Electron Microscopy II: Specific Ultrastructural Probes

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1974; KOEHLER and PERKINS, 1974; KOEHLER, 1976). Radioiodine is another antibody marker for TEM. Its presence can be detected after radioautographic procedures as electron-opaque silver grains. One distinct advantage that radioiodine has over most other TEM antibody labels is that it can also be used for light-microscopic as well as TEM study. Ultrastructurally, it has a lower resolution than some of the other markers. C. Methods for Coupling Label to Antibody Once an appropriate marker has been selected for antibody coupling, a method for conjugation must be determined.

III. ). Biochim. Biophys. , Kostif, J. : Studies on phytohemagglutinins. II. Phytohemagglutinins of Pisum sativum L. and Lens esculenta moench: Specific interactions with carbohydrates. Experentia 25,17 -19 (1969) Etzler, M. , Kabat, E. : Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus. , Leseney, A. : Isolation and properties of an anti-AI phytohaemagglutinin from Dolichos biflorus seeds. Biochim. Biophys. : Electron microscope study of the fixation of lectins labelled with Raifort peroxidase on human embryonic cells 30 G.

Physiol. 42 A, 269 - 276 (1972) Isselbacher, K. : Increased uptake of amino acids and 2-deoxY-D-glucose by virustransformed cells in culture. Proc. Natl. Acad. Sci. S. , Greaves, M. : Lymphocyte activation. I. Response of T and B lymphocytes to phytomitogens. Clin. Exp. Immunol. 9,483 - 498 (1971) Kabat, E. : A study of the purification and properties of ricin. J. BioI. Chem. 168,629 - 639 (1947) Kalb, A. : The separation of three L-fucose binding proteins of Lotus tetragonolobus. Biochim. Biophys.

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